Natisha Borra
Tuesday, July 12, 2011
Molecular Biology Lab Question HELP!?
Starting with 0.5nanograms of a plasmid containing a 2KB insert in the 3KB pBluescript vector in a 50ul reaction. Using the reverse and forward primers, how many cycles will it take to make enough DNA to see on the gel (~50 nanograms in 5ul)?
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